Genetics Services offers two main services: macaque MHC genotyping and SIV viral sequencing. High-resolution MHC genotyping assays for rhesus, cynomolgus, and pig-tailed macaques constitute the bulk of our requests. Whole genome sequencing of SIV virus stocks and clinical isolates is also available through Genetics Services. We are particularly experienced in Illumina sequencing of SIVmac239, SIVmac251, and SIVsmE660 isolates.
- Roger Wiseman, Ph.D.
- Phone: (608) 890-0847
Send email inquiry about MHC genotyping
- Shelby O'Connor, Ph.D.
- Phone: (608) 890-0843
Send email inquiry about SIV sequencing
Frequently Asked Questions
How much do you charge for MHC genotyping? The cost varies depending on the type of genotyping assay used, which is determined by the species and geographic origin of the animals being typed. Please see the attached white paper or contact Genetics Services for a detailed price estimate.
What types of samples do I need to provide for MHC genotyping? The sample requirements vary by assay, but MHC genotyping assays can generally be performed with pre-isolated genomic DNA or previously prepared cDNA shipped overnight at room temperature, fresh blood samples collected in 2ml EDTA tubes and shipped overnight with cold packs, or cryopreserved PBMC shipped overnight on dry ice.
What is the turnaround time for MHC genotyping? This varies on the number of samples in the queue, but is usually between 8 and 12 weeks. Priority turnaround may be available at extra charge.
Do you sequence viruses other than SIV? Or more exotic SIV strains? We have validated our whole genome assays on SIVmac239, SIVmac251, and SIVsmE660. New assays can be designed for other SIV strains. Genetics Services colleagues also have experience sequencing other RNA viruses including dengue virus and hepatitis C virus. The availability of sequencing for these these and other non-SIV viruses will be determined on a case-by-case basis.
What is the minimum viral load necessary for SIV sequencing? SIV sequencing is most reliable when there are at least 1,000 virions in the sample.
last updated July 29, 2016