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Training 

Click here for more information about the 2011 NHP MHC class I genotyping and allele discovery workshop. The workshop will be held November 7-12, 2011 in Madison, WI.


Researcher Feedback 


Available NHP MHC reagents 


Laboratory Protocols 

Novel major histocompatibility complex (MHC) allele discovery

• from cDNA libraries by Sanger sequencing

MHC class I

MHC class II

• from cDNA amplicons by Sanger sequencing

MHC class I

MHC class II

• from cDNA amplicons by Roche/454 pyrosequencing

MHC class I

MHC class II

Major histocompatibility complex genotyping

PCR-SSP for 29 common Indian rhesus macaque MHC class I alleles

PCR-SSP for 8 common Chinese rhesus macaque MHC class I alleles

PCR-SSP for 4 common Mauritian cynomolgus macaque MHC class I alleles

• Roche/454 pyrosequencing for rhesus, cynomolgus, and pig-tailed macaques

• FLX chemistry

Titanium chemistry

• DNA microsatellite haplotyping for rhesus, cynomolgus, and pig-tailed macaques

UW-Madison 16 marker panel

• Biomedical Primate Research Center XX marker panel

 

  Attached Files  
   
 UW MHC Microsatellite Haplotyping.doc


Files 

MHC Contract Web Portal Announcements 
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Researcher feedback is now live
doconnor 2010-05-26 11:51
External researchers: good news, the feedback link is now live. Interested researchers can request biological reagents, ask for technical support on protocols, and leave feedback on the MHC contract web portal.
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Welcome to the MHC Contract Web Portal
doconnor 2010-04-30 16:45
Welcome to the MHC Contract Web Portal (MCWP). The MCWP is a repository for data collected under NIH NIAID contract HHSN266200400088C, awarded to the University of Wisconsin-Madison.
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