PCR-SSP primers for 8 common Chinese rhesus macaque MHC class I alleles
| Allele | Primer Sequence | Concentration | |
| Mamu-A1*2601 | 5' | GAG AGC CTG CGG AAC CTC | 0.5 μM |
| 3' | GCT TCC ATG TCA GTG TGA TCC | ||
| Mamu-A1*5702 | 5' | CAT GTG ACC CAC CAC CCT | 0.5 μM |
| 3' | CTC TTC CTC CTC CAC ATC ACA A | ||
| Mamu-B*1702 | 5' | AAC GGT GAC CTG CGC TCT | 0.5 μM |
| 3' | CTC TTC TCC AGA AGG CAC CAA | ||
| Mamu-B*3901 | 5' | GAC GTC GAG AGT CCG AGG T | 0.5 μM |
| 3' | CCT CAT GGT CAG AGA TGG GA | ||
| Mamu-B*8901 | 5' | GGC TGC CGG AGC CTG | 0.5 μM |
| 3' | GAC ACA CAA GAG CTC TGG GC | ||
| Mamu-B*9001 | 5' | GGG AGG CGA CCC GTT AT | 0.5 μM |
| 3' | GCC AAG CCA ACA ACA ATG G | ||
| Mamu-B*9101 | 5' | GGG TCT CAC ACC ATC CAG ATA | 0.5 μM |
| 3' | TAG GGT GAG GGG CTC CA | ||
| Mamu-B*1902 | 5' | GAG GGG GGG TCT CAC ACA G | 0.5 μM |
| 3' | GGT GAC AAC TGC TAG GAC AGC A |
*The annealing temperature used to amplify Mamu-B*1902 was 69°C, while the annealing temperature used to amplify the remaining 7 alleles was 65°C.
Reference:
Karl JA, et al. Identification of MHC class I sequences in Chinese-origin rhesus macaques. Immunogenetics. 2008 Jan;60(1):37-46. Epub 2007 Dec 21.
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