Novel major histocompatibility complex (MHC) allele discovery

• from cDNA libraries by Sanger sequencing

• MHC class I

• MHC class II

• from cDNA amplicons by Sanger sequencing

• MHC class I

• MHC class II

• from cDNA amplicons by Roche/454 pyrosequencing

• MHC class I

• MHC class II

Major histocompatibility complex genotyping

• PCR-SSP for 29 common Indian rhesus macaque MHC class I alleles

• PCR-SSP for 8 common Chinese rhesus macaque MHC class I alleles

• PCR-SSP for 4 common Mauritian cynomolgus macaque MHC class I alleles

• Roche/454 pyrosequencing for rhesus, cynomolgus, and pig-tailed macaques

• FLX chemistry

• Titanium chemistry

• DNA microsatellite haplotyping for rhesus, cynomolgus, and pig-tailed macaques

• UW-Madison 16 marker panel

• Biomedical Primate Research Center XX marker panel