| Allele | Primer Sequence | Concentration | |
| B*430101 | 5' | CGC ACT CCA TGA GGT ATT TCA C | 0.5 μM |
| 3' | CCC TCC TGC TCC ATC CAC T | ||
| B*440101 | 5' | GAG CCC TGG TAT CTC GAA GTT | 0.5 μM |
| 3' | GGT GAG TCT GTG CGG TGA | ||
| B*460101 | 5' | GGG AGC CCC GCT TCA C | 0.5 μM |
| 3' | CCA TCT CCT GCT GGC CTA | ||
| B*510101 | 5' | CAA GGA CGC CGC ACA GT | 0.5 μM |
| 3' | GAT ACC CGC GGA GGA GGT |
*The annealing temperature used to amplify B*430101 was 69°C, while the annealing temperature used to amplify the other three alleles was 67°C.
Reference:
Mee ET, et al. MHC haplotype frequencies in a UK breeding colony of Mauritian cynomolgus macaques mirror those found in a distinct population from the same geographic origin. J Med Primatol. 2009 Feb;38(1):1-14. Epub 2008 Nov 5.
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